హరి కృష్ణ*
RP-HPLC ?????? ?????? ??????????????? ???????? ????? ?????????? ????? ????? ????? ???? ?? ????? ?????? ??????????????. ???????? ???????? (0.1% v/v ????????????? pH 4.5 0.1% v/v ??????????????? ???????)?? ?????? ??????? ????? ?????? ?? ???????? ???????????? ???? C18 (250 × 4.6 ????, 5 μm) ??????? ????????? ???????????????? ??????? ???????. 1 ml/min ??????? ?????? ??????? ????????? ????? ??????? HPLC ??? ????????, ???????? ???????? 2695 HPLC ????????? ????????? PDA ??????????? ???????????? 254 nm ???? ????????? ????????. ?????????? ???? 12 ????????. ??????????????? ????? ?????????? ???? ???????? ???? ?????? 3.02 ????? 6.10 ????????. ???????? ??????? ????? ??????? ????????????????? 0.999 ????? ??????? ????? 20-70 μg/ml ????? ???????????? ?????? 20-70 μg/m. ?????????? ???? ?????? ?????? 99.8% ????? ??????????????? ????? ?????????? ???? 99.8% ?? ????????????. ??????????????? 2.95 ????? 9.96 ???? LOD ????? LOQ. ?????????? 3.34 ????? 10.05 ???? LOD ????? LOQ.