నైరూప్య

Physical Control of Streptococcus pyogenes and Proteus mirabilis Using He-Ne Laser

Pohekar P.G.,Rathod S.M., Sinha A.

Microbiology is the science which deals with study of microorganisms. Some of them are beneficial and important with respect to industrial production. However most of the microorganisms are pathogen and cause the disease in human. There are several ways to control the population of microorganism including the use of antibiotics at the top; however the LASER (Light Amplification by Stimulated Emission of Radiation) irradiation also provides the effective way to control the microbial population. The bacteria selected were include both Gram positive i.e. Streptococcus pyogenes and Gram negative i.e. Proteus mirabilis. These bacteria are exposed to the 632.8nm He-Ne laser beam in order to study the effect. The increase in the exposure time of the radiation resulted in the reduction of the bacterial count due to their destruction because of the radiation. For Streptococcus pyogenes, the best results shows at 17.5 mW and 150 seconds, where as for Proteus mirabilis, the reduction occur at 17.5 mW for 150 seconds. Today LASER use in many field such as skin treatment, ulcerous would, the diabetic wounds. So once this method approve, this could be the invaluable tool to control bacteria