నైరూప్య

A non-tissue culture-dependent approach for genetic transformation of Marchantia polymorpha L.

Lan Zhang, Ning Xu, Jianming Man, Luo Rui*

Marchantia polymorpha L. is a prime example of an early terrestrial plant. It acts as a desirable model species for biological research as a result of its short growth cycle, predominant haploid gametophyte, simultaneous sexual and asexual propagation, etc. In this study, a non-tissue culture-dependent approach to the genetic transformation of M. polymorpha L. was developed. This approach comprises immersion of explants (thalli or mature gemmae obtained in the field conveniently, with no pre-culture) in the solution of Agrobacterium, inoculation on soil simply, and screening by spraying selection solution directly on them. As a result, over-expression of MpCCAAT-NFYB5 (Mapoly0053s0055.1) and corresponding phenotypic changes were observed. Furthermore, it was demonstrated that non-chimera transformants could be obtained rapidly through four cycles of gemmae reproduction. This method would facilitate further molecular biological research on this specific plant