నైరూప్య

Effect of argentum nitricum (AgNO3) in both in vivo and in vitro systems

Atanu Koner and Pallavi S. Rajput

Homeopathy is one of the branches of medicinal biology, serving the mankind over more than two centuries, and is still rare to implicate in research field. The present approach is to use this cosmos to check its effects and side effects (if any) which may or may not cause any change in prokaryotic and eukaryotic system. For this it can be considered one well known homeopathic medicine for study viz. Argentum nitricum or silver nitrate (AgNO3) which is an analogue of allopathic medicine ‘Cetrizine’. Homeopathic medicines are available in different homeopathic potency (dilutions), but for the experimental purpose, it has took into consideration crude extract (mother tincture), a safe dose (potency 30) and a higher dilution 200 of the above. Assessment of the effects of medicine was performed on two systems namely, in-vivo (in body) and in-vitro (in laboratory). In in vitro system, the study was done by considering bacteria (gram positive, gram negative) and fungi, their growth under normal condition and growth after homeopathic medicine treatment. Also molecular analysis was done by checking out the central dogma pathway. Whereas in the in vivo system, dose effectiveness was checked in eukaryotic body (genetically pure line albino rats) and the various physiological parameters were studied in relation to the interrelated organs.